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"Recombinant Protein Production in Yeast: Methods and Protocols," edited by Roslyn M. Bill, is a comprehensive guide within the Methods in Molecular Biology series. The book provides detailed methodologies for producing recombinant proteins using yeast systems, covering topics such as expression vector preparation, transformation techniques to generate high-yielding clones, optimization of experimental conditions, scale-up processes to bioreactor formats, and strategies for yeast cell disruption to facilitate protein isolation prior to purification. Each chapter includes an introduction to the topic, a list of necessary materials and reagents, step-by-step laboratory protocols, and key tips on troubleshooting and avoiding common pitfalls. This resource aims to assist scientists in adopting yeast as a host for protein production, offering authoritative and practical insights into the field.
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Edition: 1st
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Publisher: Humana Press Inc.
Publishing Year: 2012
ISBN: 9781617797699
Pages: 247